| SAG Strain Number: | 1224-1b | 
		
		| TAXONOMY | 
		| Genus: | Lepocinclis | 
		| Species: | acus | 
		| Taxonomic position: | Euglenophyta - Euglenophyceae | 
		| Authority: | (O. F. Müller) Marin et Melkonian | 
		| Variety: | maior | 
		| Formerly called: | authentic strain of Euglena acus Ehrenberg var. maior Pringsheim | 
		| Authentic: | yes | 
		| Division: | Euglenophyta | 
		| Class: | Euglenophyceae | 
		| ORIGIN | 
		| General habitat: | freshwater | 
		| Climatic zone: | temperate | 
		| Continent: | Europe | 
		| Country: | United Kingdom | 
		| Locality: | near Cambridge | 
		| Lat. / Long.  (Precision): | 52.202347 / 0.128345  (7000m)   View on Google Maps | 
| Year: | before 1954 | 
		| Isolated by: | E.G. Pringsheim | 
		| Strain number by isolator: | strain 2 as Euglena acus Ehrenberg var. maior Pringsheim | 
		| Deposition by: | E.G. Pringsheim (SAG) | 
		| Deposition date: | 1954 | 
		| CULTURE INFORMATION | 
		| Culture medium: | ErbsMS | 
		| Axenic: | bacterial or other types of contamination | 
		| Strain relatives: | UTEX 1316; CCAP 1224/1B [dec.] | 
| Agitation resistance: | not detected | 
| Special properties: | NO known Nagoya Protocol restrictions for this strain; | 
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| PUBLICATIONS | 
| Publication: | Kim,J.I. et al. (2015) Taxon-rich multigene phylogeny of photosynthetic euglenoids (Euglenophyceae). Frontiers in Ecology and Evolution 3 | 
| Publication: | Kim J.I. et al. (2014) Molecular Phylogeny and Cryptic Diversity of the Genus Phacus (Phacaceae, Euglenophyceae) and the Descriptions of Seven New Species. J. Phycol. 50 (5):948-959 | 
| Publication: | Kim, J.I. et al. (2012) Phylogenetic reappraisal of the genus Monomorphina (Euglenophyceae) based on molecular and morphological data. J. Phycol. 49 (1):82-91 | 
| Publication: | Kim J.I. et al. (2010) Multigene Analyses of Photosynthetic Euglenoids and New Family, Phacaceae (Euglenales). J. Phycol. 46 (6):1278–1287 | 
| Publication: | Linton E.W. et al. (2010) Reconstructing Euglenoid Evolutionary Relationships using Three Genes: NuclearSSU and LSU, and Chloroplast SSUrDNA Sequences and the Description of Euglenaria gen. nov. (Euglenophyta). Protist 161:603-619 | 
| Publication: | Marin, B. et al. (2003) Phylogeny and taxonomic revision of plastid-containing Euglenophytes based on SSU rDNA sequence comparisons and synapomorphic signatures in the SSU rRNA secondary structure. Protist  154 (1):99-145 | 
| Publication: | Pringsheim, E.G.  (1956) Contributions towards a monograph of the genus Euglena. Nova Acta Leopoldina N.F. 18:1-168 | 
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| SEQUENCE INFORMATION | 
| Sequence Accession: | JN603872  Lepocinclis acus var. major strain SAG1224-1b 26S large subunit ribosomal RNA gene, partial sequence.  (1035 bp) | 
| Sequence Accession: | AJ532460  Euglena acus var. major partial 18S rRNA gene, strain SAG 1224-1b. (2159 bp) | 
| Sequence Accession: | EF525351  Lepocinclis acus strain SAG1224-1b large subunit ribosomal RNA gene, partial sequence; plastid. (1771 bp) | 
		| CRYO | 
		| Cryopreservation: | 2 step: no survival 5% MetOH and DMSO | 
| TEACHING | 
		| Recommended for teaching: | no |