| SAG Strain Number: | 2063 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | ciferrii |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Negroni et Blaisten |
| Variety: | |
| Formerly called: | Prototheca zopfii Krüger var. occulta Roesler et al. |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | Germany |
| Locality: | from cattle liquid manure, Cow barn |
| Lat. / Long. (Precision): | |
| Year: | before 1995 |
| Isolated by: | R. Blaschke-Hellmessen |
| Strain number by isolator: | RZ I-3 |
| Deposition by: | U. Roesler |
| Deposition date: | 20.02.2005 |
| CULTURE INFORMATION |
| Culture medium: | TOM Ag |
| Axenic: | axenic |
| Strain relatives: | |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; thermophile (38°C). Animal pathogene. Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 2063 P. zopfii var. occulta reassigned into type strain of P. ciferrii Negroni et Blaisten 1941 by Jagielski et al. 2019. SAG 06/2022; "Biotype I " according to Roesler et al. (2003) |
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| PUBLICATIONS |
| Publication: | Kano R. et al. (2022) Phenotypic Characteristics of Prototheca Species Occurring in Humans and Animals. Med. Mycol. J. 63 (1):17-20 |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Miura A. et al. (2020) In vitro algaecid effect of itraconazole and ravuconazole on Prototheca species. Medical Mycology 58 (6):845-847 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Jagielski T. et al. (2018) The activity of silver nanoparticles against microalgae of the Prototheca genus. Nanomedicine 13 (9):1025-1036 |
| Publication: | Severgnini M. et al. (2018) Genome sequencing of Prototheca zopfii genotypes 1 and 2 provides evidence of a severe reduction in organellar genomes. Sci. Rep. 8 (1):14637 |
| Publication: | Morandi S. et al. (2017) Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk. Int. J. Syst. Evol. Microbiol. 67:3865-3871 |
| Publication: | Marques S. et al. (2015) Internal transcribed spacer sequence-based rapid molecular identification of Prototheca zopfii and Prototheca blaschkeae directly from milk of infected cows. Journal of Dairy Science 98 (5):3001-3009 |
| Publication: | Ewing A. et al. (2014) 16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes. J. Phycol. 50 (4):765-769 |
| Publication: | Hirose N. et al. (2013) Ribosomal Internal Transcribed Spacer of Prototheca wickerhamii Has Characteristic Structure Useful for Identification and Genotyping. PLOS One 8 (11):e81223 |
| Publication: | Ahrholdt J. et al. (2012) Epidemiological analysis of worldwide bovine, canine and human clinical Prototheca isolates by PCR genotyping and MALDI-TOF mass spectrometry proteomic phenotyping. Medical Mycology 50 (3):234-243 |
| Publication: | Murugaiyan J. et al. (2012) Establishment of a matrix-assisted laser desorption ionization time-offlight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca. Clin Microbiol Infect 18:461–464 |
| Publication: | Roth K. (2009) Ätiologische Bedeutung von Prototheca zopfii Genotyp 2 bei der Protothekenmastitis des Rindes. :105 |
| Publication: | Thompson G. et al. (2009) Algaemia in a dairy cow by Prototheca blaschkeae. Med. Mycol. 47:527-531 |
| Publication: | von Bergen M. et al. (2009) Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS. Proteomics Clin. Appl. 3:774–784 |
| Publication: | Aouay A. et al. (2008) Molecular characterization of Prototheca strains isolated from bovine mastitis. Journal de Mycologie Médicale 18:224—227 |
| Publication: | Marques S. et al. (2008) Bovine mastitis associated with Prototheca blaschkeae. Journal of Clinical Microbiology 46:1941-1945 |
| Publication: | Möller A. et al. (2007) Prototheca zopfii genotype 2—The causative agent of bovine protothecal mastitis?. Veterinary Microbiology 120:370–374 |
| Publication: | Roesler U. et al. (2006) Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov. Int. J. Syst. Evol. Microbiol. 56:1419-1425 |
| Publication: | Roesler, U. et al. (2003) Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Interntl. J. Syst. Evol. Microbiol. 53 (4):1195-1199 |
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| SEQUENCE INFORMATION |
| Sequencing Project: | PRJNA388740 Prototheca ciferrii strain SAG 2063, whole genome shotgun sequencing project |
| Sequence Accession: | MF163506 Prototheca ciferrii strain SAG 2063 18S ribosomal RNA gene, complete sequence (1806 bp) |
| Sequence Accession: | MF163488 Prototheca ciferrii strain SAG 2063 ITS1, 5.8S rRNA gene, and ITS2, complete sequence (912 bp) |
| Sequence Accession: | MF163464 Prototheca ciferrii strain RZI-3 cytochrome b (cytB) gene, partial cds; mitochondrial (599 bp) |
| Sequence Accession: | NC_037450 Prototheca zopfii strain SAG 2063 plastid, complete genome. (28698 bp) |
| Sequence Accession: | NC_037449 Prototheca zopfii strain SAG 2063 mitochondrion, complete genome. (38164 bp) |
| Sequence Accession: | MF197535 Prototheca zopfii strain SAG 2063 plastid, complete genome. (28698 bp) |
| Sequence Accession: | MF197533 Prototheca zopfii strain SAG 2063 mitochondrion, complete genome. (38164 bp) |
| Sequence Accession: | AY973040 Prototheca zopfii strain SAG 2063 18S ribosomal RNA gene, partial sequence. (1440 bp) |
| Sequence Accession: | KX389285 Prototheca zopfii isolate SAG-2063 beta-tubulin (TUB1) mRNA, complete cds. (1514 bp) |
| Sequence Accession: | KX389284 Prototheca zopfii isolate SAG-2063 alpha tubulin (TUA1) mRNA, complete cds. (1553 bp) |
| Sequence Accession: | KY654530 Prototheca zopfii strain SAG2063 28S ribosomal RNA gene, partial sequence. (581 bp) |
| Sequence Accession: | KX499484 Prototheca zopfii strain SAG2063 18S ribosomal RNA gene, partial sequence; mitochondrial. (1440 bp) |
| Sequence Accession: | KF983336 Prototheca zopfii strain SAG 2063 23S ribosomal RNA gene, partial sequence; chloroplast. (483 bp) |
| Sequence Accession: | KF983332 Prototheca zopfii strain SAG 2063 16S ribosomal RNA gene, partial sequence; mitochondrial. (878 bp) |
| Sequence Accession: | AB758653 Prototheca zopfii gene for 18S rRNA, partial sequence, strain: SAG 2063. (406 bp) |
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| CRYO |
| Cryopreservation: | standard 5% DMSO; very good survival |
| TEACHING |
| Recommended for teaching: | no |
