| SAG Strain Number: | 2064 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | blaschkeae |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Roesler et al. |
| Variety: | |
| Formerly called: | P. zopfii biotype III |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | Germany |
| Locality: | from human onychomykosis |
| Lat. / Long. (Precision): | |
| Year: | before 1995 |
| Isolated by: | R. Blaschke-Hellmessen |
| Strain number by isolator: | RZ III-3 |
| Deposition by: | U. Roesler |
| Deposition date: | 20.02.2005 |
| CULTURE INFORMATION |
| Culture medium: | TOM Ag |
| Axenic: | axenic |
| Strain relatives: | |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; thermophile (38°C). Human pathogene. Risk Level 2 > restricted distribution! Handle with care. |
| |
| PUBLICATIONS |
| Publication: | Kano R. et al. (2022) Phenotypic Characteristics of Prototheca Species Occurring in Humans and Animals. Med. Mycol. J. 63 (1):17-20 |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Jagielski T. et al. (2018) The activity of silver nanoparticles against microalgae of the Prototheca genus. Nanomedicine 13 (9):1025-1036 |
| Publication: | Jagielski T. et al. (2018) 3-Bromopyruvate as an Alternative Option for the Treatment of Protothecosis. Front. Pharmacol. 9:375 |
| Publication: | Morandi S. et al. (2017) Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk. Int. J. Syst. Evol. Microbiol. 67:3865-3871 |
| Publication: | Ewing A. et al. (2014) 16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes. J. Phycol. 50 (4):765-769 |
| Publication: | Ahrholdt J. et al. (2012) Epidemiological analysis of worldwide bovine, canine and human clinical Prototheca isolates by PCR genotyping and MALDI-TOF mass spectrometry proteomic phenotyping. Medical Mycology 50 (3):234-243 |
| Publication: | Jagielski, T. et al. (2012) Multicentre Etest evaluation of in vitro activity of conventional antifungal drugs against European bovine mastitis Prototheca spp. isolates. J. Antimicrob. Chemother. 67:1945-1947 |
| Publication: | Murugaiyan J. et al. (2012) Establishment of a matrix-assisted laser desorption ionization time-offlight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca. Clin Microbiol Infect 18:461–464 |
| Publication: | Marques S. et al. (2010) Short communication: Temperature sensibility of Prototheca blaschkeae strains isolated from bovine mastitic milk. Journal of Dairy Science 93 (11):5110-5113 |
| Publication: | Roth K. (2009) Ätiologische Bedeutung von Prototheca zopfii Genotyp 2 bei der Protothekenmastitis des Rindes. :105 |
| Publication: | Thompson G. et al. (2009) Algaemia in a dairy cow by Prototheca blaschkeae. Med. Mycol. 47:527-531 |
| Publication: | von Bergen M. et al. (2009) Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS. Proteomics Clin. Appl. 3:774–784 |
| Publication: | Aouay A. et al. (2008) Molecular characterization of Prototheca strains isolated from bovine mastitis. Journal de Mycologie Médicale 18:224—227 |
| Publication: | Marques S. et al. (2008) Bovine mastitis associated with Prototheca blaschkeae. Journal of Clinical Microbiology 46:1941-1945 |
| Publication: | Möller A. et al. (2007) Prototheca zopfii genotype 2—The causative agent of bovine protothecal mastitis?. Veterinary Microbiology 120:370–374 |
| Publication: | Roesler U. et al. (2006) Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov. Int. J. Syst. Evol. Microbiol. 56:1419-1425 |
| Publication: | Roesler, U. et al. (2003) Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Interntl. J. Syst. Evol. Microbiol. 53 (4):1195-1199 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | MF163449 Prototheca blaschkeae strain RZIII-3 (cytB) gene, partial cds; mitochondrial (599 bp) |
| Sequence Accession: | MF163511 Prototheca blaschkeae strain SAG 2064 18S rRNA gene, complete sequence (1816 bp) |
| Sequence Accession: | MF163498 Prototheca blaschkeae strain SAG 2064 ITS1, 5.8S rRNA gene, and ITS2, complete sequence (1198 bp) |
| Sequence Accession: | AY973041 Prototheca blaschkeae strain SAG 2064 18S ribosomal RNA gene, partial sequence. (1450 bp) |
| Sequence Accession: | KX389283 Prototheca blaschkeae isolate SAG-2064 beta-tubulin (TUB1) mRNA, complete cds. (1384 bp) |
| Sequence Accession: | KX389282 Prototheca blaschkeae isolate SAG-2064 alpha tubulin (TUA1) mRNA, complete cds. (1492 bp) |
| Sequence Accession: | KX499483 Prototheca blaschkeae strain SAG2064 18S ribosomal RNA gene, partial sequence; mitochondrial. (1451 bp) |
| Sequence Accession: | KY654532 Prototheca blaschkeae 28S ribosomal RNA gene, partial sequence (581 bp) |
| Sequence Accession: | KF983340 Prototheca blaschkeae strain SAG 2064 B 23S ribosomal RNA gene, partial sequence; chloroplast. (634 bp) |
| Sequence Accession: | KF983335 Prototheca blaschkeae strain SAG 2064 23S ribosomal RNA gene, partial sequence; chloroplast. (536 bp) |
| Sequence Accession: | KF983331 Prototheca blaschkeae strain SAG 2064 16S ribosomal RNA gene, partial sequence; mitochondrial. (875 bp) |
| Sequence Accession: | KJ374720 Prototheca blaschkeae strain SAG 2064 beta-tubulin gene, partial cds. (321 bp) |
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| CRYO |
| Cryopreservation: | not detected |
| TEACHING |
| Recommended for teaching: | no |
