| SAG Strain Number: | 211-6 |
| TAXONOMY |
| Genus: | Chlorella |
| Species: | variabilis |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Shihira et Krauss |
| Variety: | |
| Formerly called: | authentic strain of Chlorella variabilis Shihira et Krauss; C. paramecii Loefer nom. nud. |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | endosymbiont |
| Climatic zone: | |
| Continent: | America |
| Country: | USA |
| Locality: | endosymbiont of Paramecium bursaria |
| Lat. / Long. (Precision): | |
| Year: | 1934 |
| Isolated by: | J. B. Loefer |
| Strain number by isolator: | |
| Deposition by: | E. G. Pringsheim (< Hall NY 1936 < J. B. Loefer) |
| Deposition date: | 1954 |
| CULTURE INFORMATION |
| Culture medium: | ESP Ag |
| Axenic: | axenic |
| Strain relatives: | ATCC 30420 as C. protothecoides |
| Agitation resistance: | yes |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; absolute requirement of vitamine B12 and thiamine B1; unable to grow on nitrate as nitrogen source, requiring ammonium or organic nitrogen compounds; sensitive to the Chlorovirus PBCV-1; resistant to the chloroviruses CVG and ATCV-1 |
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| PUBLICATIONS |
| Publication: | Bock C., Krienitz L. et al. (2011) Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea 11 (2):293–312 |
| Publication: | Hoshina, Ryo (2011) Comments on the taxonomic treatment of Micractinium reisseri (Chlorellaceae, Trebouxiophyceae), a common endosymbiont in Paramecium. Phycological Research 59 (4):269-272 |
| Publication: | Pröschold T. et al. (2011) The systematics of Zoochlorella revisited employing an integrative approach. Environm. Microbiol. 13 (2):350–364 |
| Publication: | Hoshina R. et al. (2010) Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycol. Res. 58:188-201 |
| Publication: | Luo, W. et al. (2010) Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biol. 12 (3):545-553 |
| Publication: | Linz, B. et al. (1999) Correlation between virus-sensitivity and isoenzyme spectrum in symbiotic Chlorella-like algae. Protistology 1 (2):76-81 |
| Publication: | Huss V.A.R. et al. (1993) There is an ecological basis for host/symbiont specificity in Chlorella/Hydra symbioses. Endocyt. Cell Res. 10 (1-2):35-46 |
| Publication: | Liersch R. (1976) Über das ätherische Öl von Grünalgen. I. Die Öle der Gattung Chlorella (On the essential oil of green algae. I. The oils of the genus Chlorella). Arch. Microbiol. 107:353-356 |
| Publication: | Kessler E. (1967) Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella III. Merkmale von 8 autotrophen Arten. Archiv für Mikrobiologie 55 (4):346-357 |
| Publication: | Shihira, I. et al. (1965) Chlorella, Physiology and taxonomy of forty-one isolates. :97 pp. |
| Publication: | Loefer, B. (1936) Isolation and growth characteristics of the "zoochlorella" of Paramecium bursaria. Amer. Naturalist 70:1-4 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | FM205849 Chlorella variabilis 18S rRNA gene (partial), ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene (partial), strain SAG 211-6. (3948 bp) |
| CRYO |
| Cryopreservation: | good survival |
| TEACHING |
| Recommended for teaching: | no |
