| SAG Strain Number: | 211-7a |
| TAXONOMY |
| Genus: | Auxenochlorella |
| Species: | protothecoides |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | (Krüger) Kalina et Puncochárová |
| Variety: | |
| Formerly called: | authentic strain of Chlorella protothecoides Krüger |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | freshwater |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | Germany |
| Locality: | sap from wounded Populus alba |
| Lat. / Long. (Precision): | |
| Year: | 1892 |
| Isolated by: | W. Krüger |
| Strain number by isolator: | |
| Deposition by: | E.G. Pringsheim ( < H. Meyer (as 1930/32, St. No. 17) < W. Krüger) |
| Deposition date: | 1954 |
| CULTURE INFORMATION |
| Culture medium: | PolGlu Ag |
| Axenic: | axenic |
| Strain relatives: | CCAP211/7A; UTEX 25; ATCC 30407 |
| Agitation resistance: | yes |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; requires thiamine (vitamin B1), cannot use NO3 but e.g. NH4+ or other organics as nitrogen source |
| |
| PUBLICATIONS |
| Publication: | Perez B. et al. (2025) Microfiltration of microalgae biomolecules: Impact of pulsed electric fields and high-pressure homogenization on single-cell protein recovery and membrane fouling. Algal Res. 88:104015 |
| Publication: | Perez B. et al. (2025) Reversible electropermeabilization of Auxenochlorella protothecoides microalgae: tracking mass transfer and membrane resealing dynamics induced by pulsed electric fields. Algal Res. 90:104125 |
| Publication: | Papachristou I. et al. (2023) Direct transesterification of microalgae after pulsed electric field treatment. J. Chem. Techno.l Biotechnol. 98:642-650 |
| Publication: | Zhang Y. et al. (2022) Pulsed microwave pretreatment of fresh microalgae for enhanced lipid extraction. Energy 248 |
| Publication: | Papachristou I. et al. (2020) Evaluation of pulsed electric fields effect on the microalgae cell mechanical stability through high pressure homogenization. Algal Res. 47:101847 |
| Publication: | Guo B. et al. (2019) Hydrothermal liquefaction of residual microalgae biomass after pulsed electric field-assisted valuables extraction. Algal Res. 43:101650 |
| Publication: | Silve A. et al. (2018) Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy. Bioresource Technol. 269:179-187 |
| Publication: | Silve A. et al. (2018) Extraction of lipids from wet microalga Auxenochlorella protothecoides using pulsed electric field treatment and ethanol-hexane blends. Algal Res. 29:212-222 |
| Publication: | Straessner R. et al. (2016) icroalgae precipitation in treatment chambers during pulsed electric field (PEF) processing. Innov. Food Sci. Emerg. Technol. 37:391-399 |
| Publication: | Darienko T. et al. (2015) GENETIC VARIABILITY AND TAXONOMIC REVISION OF THE GENUS AUXENOCHLORELLA (SHIHIRA ET KRAUSS) KALINA ET PUNCOCHAROVA (TREBOUXIOPHYCEAE, CHLOROPHYTA). J. Phycol. 51:394-400 |
| Publication: | Piasecka, A. et al. (2014) Physical Methods of Microalgal Biomass Pretreatment. Int. Agrophys. 28:341-348 |
| Publication: | Fulnečková J. et al. (2013) A Broad Phylogenetic Survey Unveils the Diversity and Evolution of Telomeres in Eukaryotes. Genome Biology and Evolution 5 (3):468-483 |
| Publication: | Goettel M. et al. (2013) Pulsed electric field assisted extraction of intracellular valuables from microalgae. Algal Res. 2 (4):401-408 |
| Publication: | Fulnečková J. et al. (2012) Dynamic Evolution of Telomeric Sequences in the Green Algal Order Chlamydomonadales. Genome Biology and Evolution 4 (3):248-264 |
| Publication: | Görs M. et al. (2010) THE POTENTIAL OF ERGOSTEROL AS CHEMOTAXONOMIC MARKER TO DIFFERENTIATE BETWEEN ‘‘CHLORELLA’’ SPECIES (CHLOROPHYTA). J. Phycol. 46:1296–1300 |
| Publication: | Shinde S.D. et al. (2010) Statistical Media Optimization for Lutein Productein from Microalgae Auxenochlorella protothecoides SAG 211-7A . International Journal of Advanced Biotechnology and Research 1 (2):104-114 |
| Publication: | Nyati, S. et al. (2007) Fine structure and phylogeny of green algal photobionts in the microfilamentous genus Psoroglaena (Verrucariaceae, lichen-forming ascomycetes). Plant Biol (Stuttg) 9 (3):390-9 |
| Publication: | Cozzolino S. et al. (1999) The use of nuclear ribosomal ITS 1 DNA sequences for the identification of Chlorella strains. Algological Studies 95:31-42 |
| Publication: | Huss, V.A.R. et al. (1999) Biochemical taxonomy and molecular phylogeny of the genus Chlorella sensu lato (Chlorophyta). J. Phycol. 35:587-598 |
| Publication: | Linz, B. et al. (1999) Correlation between virus-sensitivity and isoenzyme spectrum in symbiotic Chlorella-like algae. Protistology 1 (2):76-81 |
| Publication: | Huss V.A.R. et al. (1993) There is an ecological basis for host/symbiont specificity in Chlorella/Hydra symbioses. Endocyt. Cell Res. 10 (1-2):35-46 |
| Publication: | Huss, V.A. et al. (1990) Phylogenetic position of some Chlorella species within the chlorococcales based upon complete small-subunit ribosomal RNA sequences. J. Mol. Evol. 31 (5):432-442 |
| Publication: | Kessler E. et al. (1988) Species-specific ability of Chlorella strains (Chlorophyceae) to form stable symbioses with Hydra viridis. Pl. Syst. Evol. 160:241-246 |
| Publication: | Kalina T. et al. (1987) Taxonomy of the subfamily Scotiellocystoideae Fott 1976 (Chlorellaceae, Chlorophyceae). Arch. Hydrobiol. Suppl. (Algol. Stud. 45) 73 (4):473-521 |
| Publication: | Reisser W. et al. (1986) Studies on ultrastructure and host range of a Chlorella attacking virus. Protoplasma 135 (2):162-165 |
| Publication: | Kerfin W. et al. (1978) Physiological and biochemical contributions to the taxonomy of the genus Chlorella. IX. DNA-Hybridization. Arch. Hydrobiol. 116:97-103 |
| Publication: | Kessler E. (1976) Comparative physiology, biochemistry, and the taxonomy of Chlorella (Chlorophyceae). Plant Systematics and Evolution 125 (3):129-138 |
| Publication: | Liersch R. (1976) Über das ätherische Öl von Grünalgen. I. Die Öle der Gattung Chlorella (On the essential oil of green algae. I. The oils of the genus Chlorella). Arch. Microbiol. 107:353-356 |
| Publication: | Kessler E. et al. (1971) Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella. V. Die auxotrophen und mesotrophen Arten (Physiological and biochemical contributions to the taxonomy of the genus Chlorella. V. The auxotrophic and mesotrophic species). Arch. Microbiol. 79:44-48 |
| Publication: | Kessler E. (1967) Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella III. Merkmale von 8 autotrophen Arten. Archiv für Mikrobiologie 55 (4):346-357 |
| Publication: | Krüger, W. (1894) Beiträge zur Kenntnis der Organismen des Saftflusses (sog. Schleimflusses) der Laubbäume. In: Zopf, W. [Ed.] Beitraege zur Physiologie und Morphologie Niederer Organismen |
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| SEQUENCE INFORMATION |
| Sequence Accession: | AM260464 partial 26S rRNA gene (678 bp) |
| Sequence Accession: | AM260440 chloroplast partial rbcL gene for ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit. (990 bp) |
| Sequence Accession: | AB097096 Auxenochlorella protothecoides gene for 28S rRNA, partial sequence. (583 bp) |
| Sequence Accession: | X65688 (1541 bp) |
| Sequence Accession: | X56101 18S rRNA gene (1832 bp) |
| CRYO |
| Cryopreservation: | good survival, standard 2-step protocol 5% DMSO |
| TEACHING |
| Recommended for teaching: | no |
