| SAG Strain Number: | 222-2a | 
		
		| TAXONOMY | 
		| Genus: | Chlorella | 
		| Species: | spec. | 
		| Taxonomic position: | Chlorophyta - Trebouxiophyceae | 
		| Authority: |  | 
		| Variety: |  | 
		| Formerly called: | Dictyosphaerium pulchellum Wood (Chlorophyta - Chlorophyceae) | 
		| Authentic: | no | 
		| Division: | Chlorophyta | 
		| Class: | Trebouxiophyceae | 
		| ORIGIN | 
		| General habitat: | freshwater | 
		| Climatic zone: |  | 
		| Continent: | Europe | 
		| Country: | France | 
		| Locality: | near Amies | 
		| Lat. / Long.  (Precision): |  | 
| Year: | 1949 | 
		| Isolated by: | E.A. George | 
		| Strain number by isolator: |  | 
		| Deposition by: | E.G. Pringsheim (SAG) | 
		| Deposition date: | 1954 | 
		| CULTURE INFORMATION | 
		| Culture medium: | ESP Ag | 
		| Axenic: | axenic | 
		| Strain relatives: | CCAP 222/2A; UTEX 70; ATCC 30415 | 
| Agitation resistance: | not detected | 
| Special properties: | NO known Nagoya Protocol restrictions for this strain; transferred from Dictyosphaerium pulchellum Wood (Chlorophyceae) to Chlorella spec. (Trebouxiophyceae) acc. to molecular data (e.g. Krienitz et al. 2010) | 
|   | 
| PUBLICATIONS | 
| Publication: | Krivina E. et al. (2024) A description of Aliichlorella ignota gen. et sp. nov. and a comparison of the efficiency of species delimitation methods in the Chlorella-clade (Trebouxiophyceae, Chlorophyta). Phycol. Res. 72:180-190 | 
| Publication: | Alexandrou, M.A. et al. (2015) Evolutionary relatedness does not predict competition and co-occurrence in natural or experimental communities of green algae. Proc. Royal Soc. B 282 (1799) | 
| Publication: | Bock C., Krienitz L. et al. (2011) Taxonomic reassessment of the genus Chlorella (Trebouxiophyceae) using molecular signatures (barcodes), including description of seven new species. Fottea 11 (2):293–312 | 
| Publication: | Görs M. et al. (2010) THE POTENTIAL OF ERGOSTEROL AS CHEMOTAXONOMIC MARKER TO DIFFERENTIATE BETWEEN ‘‘CHLORELLA’’ SPECIES (CHLOROPHYTA). J. Phycol. 46:1296–1300 | 
| Publication: | Krienitz L. et al. (2010) Polyphyletic origin of the Dictyosphaerium morphotype within Chlorellaceae (Trebouxiophyceae). J. Phycol. 46 (3):559-563 | 
| Publication: | Luo, W. et al. (2010) Generic concept in Chlorella-related coccoid green algae (Chlorophyta, Trebouxiophyceae). Plant Biol. 12 (3):545-553 | 
| Publication: | Pröschold, T. et al. (2010) Polyphyletic distribution of bristle formation in Chlorellaceae: Micractinium, Diacanthos, Didymogenes and Hegewaldia gen. nov. (Trebouxiophyceae, Chlorophyta). Phycol. Res. 58 (1):1-8 | 
| Publication: | Krienitz,L. et al. (2004) Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae). Phycologia  43 (5):529-542 | 
| show more publications ... | 
| SEQUENCE INFORMATION | 
| Sequencing Project: | SAMN02639763  Transcriptome Sequences for Mucidosphaerium pulchellum | 
| Sequence Accession: | AY323838  Mucidosphaerium pulchellum strain SAG 222-2a 18S ribosomal RNA gene, partial sequence.  (1751 bp) | 
| Sequence Accession: | AY323469  Mucidosphaerium pulchellum strain SAG 222-2a internal transcribed spacer 2, complete sequence.  (242 bp) | 
| Sequence Accession: | FM205857  Chlorella sp. SAG 222-2a 18S rRNA gene (partial), ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene (partial), strain SAG 222-2a. (3763 bp) | 
		| CRYO | 
		| Cryopreservation: | good survival | 
| TEACHING | 
		| Recommended for teaching: | no |