| SAG Strain Number: | 2290 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | bovis |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Jagielski et al. |
| Variety: | |
| Formerly called: | Prototheca zopfii Krüger var. hydrocarbonea |
| Authentic: | no |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | Portugal |
| Locality: | Vila do Conde, Macieira, milk from cow Bos taurus with chronic bovine protothecal mastitis |
| Lat. / Long. (Precision): | 41.342709 / -8.665506 (1000m) View on Google Maps |
| Year: | October 2002 |
| Isolated by: | S. Marques |
| Strain number by isolator: | UP-PT-P1 |
| Deposition by: | S. Marques |
| Deposition date: | 09.2008 |
| CULTURE INFORMATION |
| Culture medium: | TOM Ag |
| Axenic: | axenic |
| Strain relatives: | |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; thermophile (38°C). Animal pathogene. Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 2290 Prototheca zopfii Krüger var. hydrocarbonea reassigned into P. bovis sp. nov. Jagielski et al. 2019. SAG 06/2022 |
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| PUBLICATIONS |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Figueroa-Martinez F. et al. (2015) When the lights go out: the evolutionary fate of free-living colorless green algae. New Phytologist 206 (3):972-982 |
| Publication: | Marques S. et al. (2015) Internal transcribed spacer sequence-based rapid molecular identification of Prototheca zopfii and Prototheca blaschkeae directly from milk of infected cows. Journal of Dairy Science 98 (5):3001-3009 |
| Publication: | Marques S. et al. (2008) Bovine mastitis associated with Prototheca blaschkeae. Journal of Clinical Microbiology 46:1941-1945 |
| show more publications ... |
| SEQUENCE INFORMATION |
| Sequence Accession: | MK452815 Prototheca bovis strain SAG 2290 from Portugal cytochrome b (cytB) gene, partial cds; mitochondrial (599 bp) |
| Sequence Accession: | MK445199 Prototheca zopfii genotype 2 strain SAG 2290 small subunit ribosomal RNA gene, partial sequence. (1808 bp) |
| Sequence Accession: | MK445163 Prototheca zopfii genotype 2 strain SAG 2290 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, parti (853 bp) |
| Sequence Accession: | FR848894 tRNA-Ile gene, tRNA-Ala gene and 23S rRNA gene (partial), strain UP-PT-P1. (2085 bp) |
| Sequence Accession: | EU439263 18S ribosomal RNA gene, partial sequence. (1807 bp) |
| CRYO |
| Cryopreservation: | standard 5% DMSO; very good survival |
| TEACHING |
| Recommended for teaching: | no |
