 
  
  | SAG Strain Number: | 2291 | 
| TAXONOMY | |
| Genus: | Prototheca | 
| Species: | blaschkeae | 
| Taxonomic position: | Chlorophyta - Trebouxiophyceae | 
| Authority: | Roesler et al. | 
| Variety: | |
| Formerly called: | P. zopfii biotype III | 
| Authentic: | no | 
| Division: | Chlorophyta | 
| Class: | Trebouxiophyceae | 
| ORIGIN | |
| General habitat: | |
| Climatic zone: | temperate | 
| Continent: | Europe | 
| Country: | Portugal | 
| Locality: | Vila do Conde, Macieira, milk from cow Bos taurus with chronic bovine protothecal mastitis | 
| Lat. / Long. (Precision): | 41.342709 / -8.665506 (1000m) View on Google Maps | 
| Year: | October 2002 | 
| Isolated by: | S. Marques | 
| Strain number by isolator: | UP-PT-P2 | 
| Deposition by: | S. Marques | 
| Deposition date: | 09.2008 | 
| CULTURE INFORMATION | |
| Culture medium: | TOM Ag | 
| Axenic: | axenic | 
| Strain relatives: | |
| Agitation resistance: | not detected | 
| Special properties: | colourless; thermophile (38°C). Animal pathogene. Risk Level 2 > restricted distribution! Handle with care. | 
| PUBLICATIONS | |
| Publication: | Figueroa-Martinez F. et al. (2015) When the lights go out: the evolutionary fate of free-living colorless green algae. New Phytologist 206 (3):972-982 | 
| Publication: | Marques S. et al. (2015) Internal transcribed spacer sequence-based rapid molecular identification of Prototheca zopfii and Prototheca blaschkeae directly from milk of infected cows. Journal of Dairy Science 98 (5):3001-3009 | 
| Publication: | Thompson G. et al. (2009) Algaemia in a dairy cow by Prototheca blaschkeae. Med. Mycol. 47:527-531 | 
| Publication: | Marques S. et al. (2008) Bovine mastitis associated with Prototheca blaschkeae. Journal of Clinical Microbiology 46:1941-1945 | 
| SEQUENCE INFORMATION | |
| Sequence Accession: | HG515045 Prototheca blaschkeae 18S rRNA gene (partial), ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene (partial), strain UP-PT-P2 (1561 bp) | 
| Sequence Accession: | EU439262 18S ribosomal RNA gene, partial sequence. (1815 bp) | 
| CRYO | |
| Cryopreservation: | not detected | 
| TEACHING | |
| Recommended for teaching: | no | 
