| SAG Strain Number: | 263-2 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | moriformis |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Krüger 1894 |
| Variety: | |
| Formerly called: | P. spec.; probably authentic strain of Prototheca moriformis Krüger |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | |
| Continent: | |
| Country: | |
| Locality: | from sap of a wounded linden tree |
| Lat. / Long. (Precision): | |
| Year: | before 1894 |
| Isolated by: | probably W. Krüger |
| Strain number by isolator: | probably authentic strain of Prototheca moriformis Krüger |
| Deposition by: | CBS Delft NL to E.G. Pringsheim |
| Deposition date: | 1948 |
| CULTURE INFORMATION |
| Culture medium: | Malz Ag |
| Axenic: | axenic |
| Strain relatives: | UTEX 288 as Prototheca moriformis (unclear, see Ewing et al. 2014); CCAP 263/2 (dec.) |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; potentially pathogenic! Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 263-2 Prototheca spec. reassigned into type strain of P. moriformis Krüger 1894 acc. to Jagielski et al. 2019. SAG 06/2022; Ewing et al (2014) found this strain to be multialgal and isolated two different strains for their molecular analysis. |
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| PUBLICATIONS |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Ewing A. et al. (2014) 16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes. J. Phycol. 50 (4):765-769 |
| Publication: | Kerfin W. et al. (1978) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca II. Starch Hydrolysis and Base Composition of DNA. Arch. Microbiol. 116 (1):105-107 |
| Publication: | Kessler E. (1977) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca I. Hydrogenase, Acid Tolerance, Salt Tolerance, Thermophily, and Liquefaction of Gelatin. Arch. Microbiol. 113:139-141 |
| Publication: | Pringsheim, E.G. (1963) Farblose Algen. Ein Beitrag zur Evolutionsforschung. Farblose Algen. Ein Beitrag zur Evolutionsforschung |
| Publication: | Krüger, W. (1894) Beiträge zur Kenntnis der Organismen des Saftflusses (sog. Schleimflusses) der Laubbäume. In: Zopf, W. [Ed.] Beitraege zur Physiologie und Morphologie Niederer Organismen |
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| SEQUENCE INFORMATION |
| Sequence Accession: | MK445189 Prototheca moriformis strain SAG 263-2 from Germany small subunit rRNA gene, partial sequence (1808 bp) |
| Sequence Accession: | MK445153 Prototheca moriformis strain SAG 263-2 from Germany SSU rRNA gene, partial sequence; ITS1, 5.8S rRNA gene, and ITS2, complete sequence; and LSU rRNA gene, partial sequence (867 bp) |
| Sequence Accession: | MK452804 UNVERIFIED: Prototheca moriformis strain UTEX 288 from Germany cytB gene, partial sequence; mitochondrial (600 bp) |
| CRYO |
| Cryopreservation: | not detected |
| TEACHING |
| Recommended for teaching: | no |
