| SAG Strain Number: | 263-4 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | pringsheimii |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Jagielski et al. |
| Variety: | |
| Formerly called: | Prototheca zopfii Krüger; authentic strain of Prototheca portoricensis var. trispora Ashford, Ciferri et Dalmau |
| Authentic: | no |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | |
| Continent: | |
| Country: | |
| Locality: | from human intestine |
| Lat. / Long. (Precision): | |
| Year: | before 1930 |
| Isolated by: | B. K. Ashford |
| Strain number by isolator: | |
| Deposition by: | |
| Deposition date: | |
| CULTURE INFORMATION |
| Culture medium: | Malz Ag |
| Axenic: | axenic |
| Strain relatives: | UTEX 327 |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; thermophile (38°C). human pathogene. Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 263-4 Prototheca zopfii Krüger reassigned as P. pringsheimii sp. nov. acc. to Jagielski et al. 2019. SAG 06/2022 |
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| PUBLICATIONS |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Ewing A. et al. (2014) 16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes. J. Phycol. 50 (4):765-769 |
| Publication: | Murugaiyan J. et al. (2012) Establishment of a matrix-assisted laser desorption ionization time-offlight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca. Clin Microbiol Infect 18:461–464 |
| Publication: | von Bergen M. et al. (2009) Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS. Proteomics Clin. Appl. 3:774–784 |
| Publication: | Roesler U. et al. (2006) Diversity within the current algal species Prototheca zopfii: a proposal for two Prototheca zopfii genotypes and description of a novel species, Prototheca blaschkeae sp. nov. Int. J. Syst. Evol. Microbiol. 56:1419-1425 |
| Publication: | Roesler, U. et al. (2003) Emended phenotypic characterization of Prototheca zopfii: a proposal for three biotypes and standards for their identification. Interntl. J. Syst. Evol. Microbiol. 53 (4):1195-1199 |
| Publication: | Roesler U. et al. (2001) Immunodiagnostic identification of dairy cows infected with Prototheca zopfii at various clinical stages and discrimination between infected and uninfected cows. J. Clin. Microbiol. 39:539-543 |
| Publication: | Kerfin W. et al. (1978) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca II. Starch Hydrolysis and Base Composition of DNA. Arch. Microbiol. 116 (1):105-107 |
| Publication: | Kessler E. (1977) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca I. Hydrogenase, Acid Tolerance, Salt Tolerance, Thermophily, and Liquefaction of Gelatin. Arch. Microbiol. 113:139-141 |
| Publication: | Pringsheim, E.G. (1963) Farblose Algen. Ein Beitrag zur Evolutionsforschung. Farblose Algen. Ein Beitrag zur Evolutionsforschung |
| Publication: | Ciferri, O. (1956) Thiamine-deficiency of Prototheca, a Yeast-like Achloric Alga. Nature 178:1475 - 147 |
| Publication: | Ashford B.K. et al. (1930) A new species of Prototheca and a variety of the same isolated from human intestine. Arch. Protistenk. 70:619-638 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | MK452820 Prototheca pringsheimii strain SAG 263-4 from Puerto Rico cytochrome b (cytB) gene, partial cds; mitochondrial (599 bp) |
| Sequence Accession: | MK445168 Prototheca pringsheimii strain SAG 263-4 from Puerto Rico SSU rRNA gene, partial sequence; ITS1, 5.8S rRNA gene, and ITS2, complete sequence; and LSU rRNA gene, partial sequence (859 bp) |
| Sequence Accession: | MK445204 Prototheca pringsheimii strain SAG 263-4 from Puerto Rico SSU rRNA gene, partial sequence (1807 bp) |
| CRYO |
| Cryopreservation: | standard 5% DMSO; very good survival |
| TEACHING |
| Recommended for teaching: | no |
