| SAG Strain Number: | 263-6 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | pringsheimii |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Jagielski et al. |
| Variety: | |
| Formerly called: | Prototheca zopfii Krüger; authentic strain of Prototheca krügeri Beijerinck ? |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | |
| Continent: | |
| Country: | |
| Locality: | from Xanthoria |
| Lat. / Long. (Precision): | |
| Year: | before 1912 |
| Isolated by: | M. W. Beijerinck |
| Strain number by isolator: | as colourless mutant of Chlorella variegata ? |
| Deposition by: | CBS Delft NL to E.G. Pringsheim |
| Deposition date: | 1948 |
| CULTURE INFORMATION |
| Culture medium: | Malz Ag |
| Axenic: | axenic |
| Strain relatives: | UTEX 329 as Prototheca kruegeri; CCAP 263/6 (dec.) |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; potentially pathogenic! Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 263-6 Prototheca zopfii Krüger reassigned into type strain of P. pringsheimii sp. nov. Jagielski et al. 2019. SAG 06/2022 |
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| PUBLICATIONS |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Kerfin W. et al. (1978) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca II. Starch Hydrolysis and Base Composition of DNA. Arch. Microbiol. 116 (1):105-107 |
| Publication: | Kessler E. (1977) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca I. Hydrogenase, Acid Tolerance, Salt Tolerance, Thermophily, and Liquefaction of Gelatin. Arch. Microbiol. 113:139-141 |
| Publication: | Pringsheim, E.G. (1963) Farblose Algen. Ein Beitrag zur Evolutionsforschung. Farblose Algen. Ein Beitrag zur Evolutionsforschung |
| Publication: | Beijerinck, M.W. (1912) Mutation bei Mikroben. In: Beijerinck, M.W. et al. [Eds.] Folia microbiologica: Holländische Beiträge zur gesamten Mikrobiologie I |
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| SEQUENCE INFORMATION |
| Sequence Accession: | MK452821 Prototheca pringsheimii strain SAG 263-6 from Netherlands cytochrome b (cytB) gene, partial cds; mitochondrial (599 bp) |
| Sequence Accession: | MK445205 Prototheca sp. ZB-2019d strain SAG 263-6 small subunit ribosomal RNA gene, partial sequence. (1806 bp) |
| Sequence Accession: | MK445169 Prototheca sp. ZB-2019d strain SAG 263-6 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial s (865 bp) |
| CRYO |
| Cryopreservation: | standard 5% DMSO; very good survival |
| TEACHING |
| Recommended for teaching: | no |
