| SAG Strain Number: | 263-7 |
| TAXONOMY |
| Genus: | Prototheca |
| Species: | ciferrii |
| Taxonomic position: | Chlorophyta - Trebouxiophyceae |
| Authority: | Negroni et Blaisten |
| Variety: | |
| Formerly called: | Prototheca zopfii Krüger |
| Authentic: | no |
| Division: | Chlorophyta |
| Class: | Trebouxiophyceae |
| ORIGIN |
| General habitat: | |
| Climatic zone: | |
| Continent: | |
| Country: | |
| Locality: | |
| Lat. / Long. (Precision): | |
| Year: | 1952 |
| Isolated by: | probably W. Krüger |
| Strain number by isolator: | as IV-7.3.2.1 |
| Deposition by: | M. B. Allen |
| Deposition date: | |
| CULTURE INFORMATION |
| Culture medium: | Malz Ag |
| Axenic: | axenic |
| Strain relatives: | UTEX 1435 (Ewing et al. 2014 found 16s and 23 s data not to be identical between UTEX 1435 and SAG 263-7); ATCC 16525 both as Prototheca moriformis |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain; colourless; potentially pathogenic! Risk Level 2 > restricted distribution! Handle with care!; non-authentic strain SAG 263-7 P. zopfii reassigned into non-authentic strain of P. ciferrii Negroni et Blaisten 1941 acc. to Jagielski et al. 2019. SAG 06/2022 |
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| PUBLICATIONS |
| Publication: | Plieger T. et al. (2022) 18S and ITS2 rDNA sequence-structure phylogeny of Prototheca (Chlorophyta, Trebouxiophyceae). Biologia 77:569-582 |
| Publication: | Dziurzynski M. et al. (2021) Prototheca-ID: a web-based application for molecular identification of Prototheca species. Database 2021:baab073 |
| Publication: | Jagielski T. et al. (2019) The genus Prototheca (Trebouxiophyceae, Chlorophyta) revisited: Implications from molecular taxonomic studies. Algal Res. 43:101639 |
| Publication: | Ewing A. et al. (2014) 16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes. J. Phycol. 50 (4):765-769 |
| Publication: | Wirth H. et al. (2012) MALDI-typing of infectious algae of the genus Prototheca using SOM portraits. Journal of Microbiological Methods 88:83–97 |
| Publication: | von Bergen M. et al. (2009) Identification of harmless and pathogenic algae of the genus Prototheca by MALDI-MS. Proteomics Clin. Appl. 3:774–784 |
| Publication: | Kerfin W. et al. (1978) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca II. Starch Hydrolysis and Base Composition of DNA. Arch. Microbiol. 116 (1):105-107 |
| Publication: | Kessler E. (1977) Physiological and Biochemical Contributions to the Taxonomy of the Genus Prototheca I. Hydrogenase, Acid Tolerance, Salt Tolerance, Thermophily, and Liquefaction of Gelatin. Arch. Microbiol. 113:139-141 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | MK452795 UNVERIFIED: Prototheca ciferrii strain SAG 263-7 cytB gene, partial sequence; mitochondrial (601 bp) |
| Sequence Accession: | MK445181 Prototheca zopfii genotype 1 strain SAG 263-7 small subunit ribosomal RNA gene, partial sequence. (1806 bp) |
| Sequence Accession: | MK445145 Prototheca zopfii genotype 1 strain SAG 263-7 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, part (905 bp) |
| CRYO |
| Cryopreservation: | standard 5% DMSO; very good survival |
| TEACHING |
| Recommended for teaching: | no |
