| SAG Strain Number: | 63-2b |
| TAXONOMY |
| Genus: | Polytomella |
| Species: | caeca |
| Taxonomic position: | Chlorophyta - Chlorophyceae |
| Authority: | Pringsheim |
| Variety: | |
| Formerly called: | authentic strain of var. minor Pringsheim |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Chlorophyceae |
| Common name: | Green Algae - Chlorophytes |
| ORIGIN |
| General habitat: | freshwater |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | Czech Republic |
| Locality: | Bohemia, mud from meadow ditch near Hirschberg (Doksy) |
| Lat. / Long. (Precision): | 50.565385 / 14.652558 (4000m) View on Google Maps |
| Year: | 1937 |
| Isolated by: | E. G. Pringsheim |
| Strain number by isolator: | strain 2 as P. caeca var. minor |
| Deposition by: | E.G. Pringsheim (SAG) |
| Deposition date: | 1954 |
| CULTURE INFORMATION |
| Culture medium: | ErbsMS |
| Axenic: | bacterial or other types of contamination |
| Strain relatives: | CCAP 63/2B |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain |
| |
| PUBLICATIONS |
| Publication: | MacDonald, S. M. et al. (2016) A survey of Polytomella (Chlorophyceae, Chlorophyta) strains in public culture collections. J Phycol 52 (4):656-663 |
| Publication: | Smith D.R. et al. (2011) Nucleotide diversity of the colorless green alga Polytomella parva (Chlorophyceae, Chlorophyta): high for the mitochondrial telomeres, surprisingly low everywhere else. J. Eukaryot. Microbiol. 58 (5):471-473 |
| Publication: | Lewis E. et al. (1974) Life Cycle of Polytomella caeca (Phytomonadida, Polyblepharidae). J. Eucaryotic Mircobiol. 21 (5):647-649 |
| Publication: | Wise D.L. (1964) The Effect of pH on the Availability of Acid Substrates for Polytomella caeca with Unexpected Activity by Succinate. J. Protozool 11:246-249 |
| Publication: | Wise D.L. (1959) Carbon Nutrition and Metabolism of Polytomella caeca. J. Protozool 6:19-23 |
| Publication: | Pringsheim, E.G. (1955) The genus Polytomella. J. Protozool. 2 (4):137-145 |
| Publication: | Wise D.L. (1955) Carbon Sources for Polytomella caeca. J. Protozool 2:156-158 |
| Publication: | Pringsheim E.G. (1936) Zur Kenntnis saprotropher Algen und Flagellaten. 2. Mitt.: Über eine neue, farblose Polyblepharidiene, Polystomella caeca. Arch. Protistenk. 88:150-160 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | HQ585011 Polytomella sp. SAG 63-2b NADH dehydrogenase subunit 2 (nad2) gene, partial cds; large subunit ribosomal RNA (rrnL8), large subunit ribosomal RNA (rrnL2), and small subunit ribosomal RNA (rrnS3) genes, complete sequence; and NADH dehydrogenase subuni (1303 bp) |
| Sequence Accession: | HQ585006 Polytomella sp. SAG 63-2b NADH dehydrogenase subunit 5 (nad5) gene, partial cds; large subunit ribosomal RNA (rrnL1), small subunit ribosomal RNA (rrnS4), large subunit ribosomal RNA (rrnL5), large subunit ribosomal RNA (rrnL4), small subunit ribosom (3825 bp) |
| Sequence Accession: | HQ585001 Polytomella sp. SAG 63-2b cytochrome b (cob) gene, partial cds; small subunit ribosomal RNA (rrnS1) and large subunit ribosomal RNA (rrnL7) genes, complete sequence; cytochrome oxidase subunit 1 (cox1) gene, complete cds; and NADH dehydrogenase subun (3246 bp) |
| Sequence Accession: | HQ584995 Polytomella sp. SAG 63-2b mitochondrial cytochrome c oxidase subunit III (cox3) gene, partial cds; nuclear gene for mitochondrial product. (1775 bp) |
| Sequence Accession: | HQ584990 Polytomella sp. SAG 63-2b 18S ribosomal RNA gene, partial sequence. (1032 bp) |
| Sequence Accession: | DQ223136 Polytomella caeca cytochrome c oxidase subunit I (cox1) gene, partial cds; mitochondrial. (546 bp) |
| Sequence Accession: | KT591081 Polytomella caeca strain SAG 63-2b cytochrome c oxidase subunit 1 (cox1) gene, complete cds; mitochondrial. (1512 bp) |
| Sequence Accession: | KT591089 Polytomella caeca strain SAG 63-2b chromosome 1 telomere; mitochondrial. (1093 bp) |
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| CRYO |
| Cryopreservation: | not detected |
| TEACHING |
| Recommended for teaching: | no |