| SAG Strain Number: | 63-4 |
| TAXONOMY |
| Genus: | Polytomella |
| Species: | magna |
| Taxonomic position: | Chlorophyta - Chlorophyceae |
| Authority: | Pringsheim |
| Variety: | |
| Formerly called: | |
| Authentic: | yes |
| Division: | Chlorophyta |
| Class: | Chlorophyceae |
| Common name: | Green Algae - Chlorophytes |
| ORIGIN |
| General habitat: | freshwater |
| Climatic zone: | temperate |
| Continent: | Europe |
| Country: | United Kingdom |
| Locality: | Cambridge, sap of elm tree in Granchester Meadows |
| Lat. / Long. (Precision): | 52.191167 / 0.103701 (1000m) View on Google Maps |
| Year: | June 1947 |
| Isolated by: | E. G. Pringsheim |
| Strain number by isolator: | strain 5 |
| Deposition by: | E.G. Pringsheim (SAG) |
| Deposition date: | 1954 |
| CULTURE INFORMATION |
| Culture medium: | ErbsMS |
| Axenic: | bacterial or other types of contamination |
| Strain relatives: | CCAP 63/3 |
| Agitation resistance: | not detected |
| Special properties: | NO known Nagoya Protocol restrictions for this strain |
| |
| PUBLICATIONS |
| Publication: | MacDonald, S. M. et al. (2016) A survey of Polytomella (Chlorophyceae, Chlorophyta) strains in public culture collections. J Phycol 52 (4):656-663 |
| Publication: | Figueroa-Martinez F. et al. (2015) When the lights go out: the evolutionary fate of free-living colorless green algae. New Phytologist 206 (3):972-982 |
| Publication: | Smith D.R. et al. (2013) Palindromic Genes in the Linear Mitochondrial Genome of the Nonphotosynthetic Green Alga Polytomella magna. Genome Biol. Evol. 5 (9):1661-1667 |
| Publication: | Smith D.R. et al. (2011) Nucleotide diversity of the colorless green alga Polytomella parva (Chlorophyceae, Chlorophyta): high for the mitochondrial telomeres, surprisingly low everywhere else. J. Eukaryot. Microbiol. 58 (5):471-473 |
| Publication: | Mallet, M.A. et al. (2006) Identification of three distinct Polytomella lineages based on mitochondrial DNA features. J. Eukaryot. Microbiol. 53 (2):79-84 |
| Publication: | Pringsheim, E.G. (1955) The genus Polytomella. J. Protozool. 2 (4):137-145 |
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| SEQUENCE INFORMATION |
| Sequence Accession: | HQ585013 Polytomella magna strain SAG 63-4 NADH dehydrogenase subunit 2 (nad2) gene, partial cds; large subunit ribosomal RNA (rrnL8), large subunit ribosomal RNA (rrnL2), and small subunit ribosomal RNA (rrnS3) genes, complete sequence; and NADH dehydrogenas (1303 bp) |
| Sequence Accession: | HQ585008 Polytomella magna strain SAG 63-4 NADH dehydrogenase subunit 5 (nad5) gene, partial cds; large subunit ribosomal RNA (rrnL1), small subunit ribosomal RNA (rrnS4), large subunit ribosomal RNA (rrnL5), large subunit ribosomal RNA (rrnL4), small subunit (3826 bp) |
| Sequence Accession: | HQ585003 Polytomella magna strain SAG 63-4 cytochrome b (cob) gene, partial cds; small subunit ribosomal RNA (rrnS1) and large subunit ribosomal RNA (rrnL7) genes, complete sequence; cytochrome oxidase subunit 1 (cox1) gene, complete cds; and NADH dehydrogena (3246 bp) |
| Sequence Accession: | HQ584997 Polytomella magna strain SAG 63-4 mitochondrial cytochrome c oxidase subunit III (cox3) gene, partial cds; nuclear gene for mitochondrial product. (1775 bp) |
| Sequence Accession: | HQ584992 Polytomella magna strain SAG 63-4 18S ribosomal RNA gene, partial sequence. (1032 bp) |
| Sequence Accession: | DQ223138 Polytomella magna cytochrome c oxidase subunit I (cox1) gene, partial cds; mitochondrial. (546 bp) |
| Sequence Accession: | KT591091 Polytomella magna strain SAG 63-4 chromosome 1 telomere; mitochondrial. (1090 bp) |
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| CRYO |
| Cryopreservation: | not detected |
| TEACHING |
| Recommended for teaching: | no |
